Novel thermosensitive hydrogel for preventing formation of abdominal adhesions

作者:Xiang Gao,Xiaohui Deng,Xiawei Wei,Hua Shi,Fengtian Wang,Tinghong Ye,Bin Shao,Wen Nie,Yuli Li,Min Luo,Changyang Gong,Ning Huang 刊名: 上传者:颜忠

【摘要】Adhesions can form after almost any type of abdominal surgery. Postoperative adhesions can be prevented by improved surgical techniques; such as reducing surgical trauma; preventing ischemia; and avoiding exposure of the peritoneal cavity to foreign materials. Although improved surgical techniques can potentially reduce formation of adhesions; they cannot be eliminated completely. Therefore; finding more effective methods to prevent postoperative adhesions is imperative. Recently; we found that a novel thermosensitive hydrogel; ie; poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) had the potential to prevent postoperative adhesions. Using the ring-opening polymerization method we prepared a PCEC copolymer which could be dissolved and assembled at 55°C into PCEC micelles with mean size of 25 nm. At body temperature; a solution containing PCEC micelles could convert into a hydrogel. The PCEC copolymer was biodegradable and had low toxicity in vitro and in vivo. We found that most animals in a hydrogel-treated group (n = 10) did not develop adhesions. In contrast; 10 untreated animals developed adhesions that could only be separated by sharp dissection (P < 0.001). The hydrogel could adhere to peritoneal wounds and degraded gradually over 7-9 days; transforming into a viscous fuid that was completely absorbed within 12 days. The injured parietal and visceral peritoneum remesothelialized over about seven and nine days; respectively. This study confirms that PCEC hydrogel has potential application in the prevention of postoperative adhesions.