衰老小鼠小脑颗粒细胞膜膜电位和动作电位改变

作者:熊杰;徐艳霞;成亮 刊名:武警医学 上传者:王小翼

【摘要】目的研究正常衰老小鼠小脑颗粒细胞膜膜电位及诱发动作电位的变化情况。方法老年(20~24月龄)和青年(3~4月龄)C57/BL6小鼠,以振荡切片法制备小脑脑切片作为观测对象,运用全细胞记录的电生理方法测定脑片上小脑颗粒细胞膜膜电位和诱发动作电位,并进行比较。结果在无任何刺激的静息条件下,衰老小鼠的小脑颗粒细胞能够维持其细胞膜电位的稳定;对于衰老神经元,诱导产生动作电位所需的内向电流降低,同时动作电位的时程延长。结论衰老虽然不影响神经元在静息条件下的状态,但影响神经元的动作电位以及其后的恢复程度:衰老神经元更容易丧失其膜电位。

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I富E圈(M。di。alJ㈣aI ofthe chinese Peopl。,。删Police F0rces)v01.16 No.08 2005—08出版 597 衰老小鼠小脑颗粒细胞膜膜电位和动作电位改变 熊 杰徐艳霞成 亮 (首都医科大学化学生物学与药学院药理室,北京100054) 摘要目的研究正常衰老小鼠小脑颗粒细胞膜膜电位及诱发动作电位的变化情况。方法老年(20~24月龄)和青 年(3~4月龄)C57/BI_6小鼠,以振荡切片法制备小脑脑切片作为观测对象,运用全细胞记录的电生理方法测定脑片上小脑颗粒细胞膜膜电位和诱发动作电位,并进行比较。结果在元任何刺激的静息条件下,衰老小鼠的小脑颗粒细胞能够维持其细胞膜电位的稳定;对于衰老神经元,诱导产生动作电位所需的内向电流降低,同时动作电位的时程延长。结论衰老虽然不影响神经元在静息条件下的状态,但影响神经元的动作电位以及其后的恢复程度:衰老神经元更容易丧失其膜电位。关键词 衰老脑切片 全细胞记录膜电位动作电位 Changes in nlcll山rane potential and£lctioIl potential of cerebellar granule lll咖ll'ons in brain secUol毽of aged C57BL/6 mice XIONG,lie,)【U.Yanxia,and CHENG Iaang.School of Chemical Biology and Pharmaceutical Sciences,Capi切I University of Medical Sciences,Beijing 100054,China Abstract Objecetlve To study the alterations in neuronal menlbvdne potential(MP)and the induced action potential(AP)in eerebel— lar granule nel//'ons in brain sections of aged mice.Methods Cerebellar sections(200 tan thickness)we/'e//lade from young(3—4 months old)and old(20—24 months old)C57BL/6 mice,and the whok—cell recording WaS used to detect the MP and the induced AP of granule nellrons.Results,111e granule neurons of aged mice could//kq/lage to maintain the MP values in resting condition.while the input—current needed to evoke a AP decreased and the duration ofAP increased remarkably in an I—clamp study.Conclusions Although aging did not show an influence Oil resting MP,it did change the AP and its recovery.Aged neurons were more vulnerable and tended to lose their MP after

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