斑马鱼Z-OTU蛋白在卵子发生和胚胎发育早期的表达(英文)

作者:岳明金;莫赛军;宋平;龚炎长 刊名:动物学研究 上传者:吴汉钧

【摘要】斑马鱼z-otu基因编码的蛋白可能具有DUBs活性,它包含OTU和TUDOR结构域,属于OTU相关蛋白酶家族的成员。该研究将原核表达的融合蛋白(OTU结构域和GST)纯化后免疫新西兰兔,获得多克隆抗体anti-Z-OTU,并利用该抗体对Z-OTU蛋白质在斑马鱼卵子发生和早期胚胎发育过程中的表达进行了分析。根据原位和整体免疫组织化学检测结果并结合以前的研究结论,分析并比较了z-otu基因的mRNA和蛋白质的分布,发现在卵子发生和早期胚胎发育过程中,z-otu基因的mRNA和蛋白质表达模式存在明显差异:mRNA仅在卵子发生早期表达,卵母细胞受精后才重新开始表达,而其蛋白在卵子发生过程中均表达;在卵子发生过程中,mRNA分布于细胞质中,而蛋白质先分布于细胞核中,然后向细胞质迁移,接着又向卵母细胞生发泡(germinal vesicle,GV)集中。推测Z-OTU蛋白类似于其他具有去泛素化酶活性的OTU相关蛋白酶,对于卵母细胞减数分裂过程中生发泡破裂、生发泡迁移及维持胚胎的分裂是必需的。

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Received date: 2010-11-08; Accepted date: 2011-04-27 Foundation items: This work was supported by grants from the ICGEB (International Center for Genetic Engineering and Biotechnology) (CRP/CHN02- 01) (SONG Ping); the National Basic Research Program of China (2004CB117400) (SONG Ping); the National Natural Science Foundation of China (30150005; 30270675) (SONG Ping) * Corresponding authors (通信作者), E-mail: pingsongps@yahoo.com.cn;gong7546@public.wh.hb.cn 收稿日期:2010-11-08;接受日期:2011-04-27 第一作者简介:岳明金,男,硕士研究生,主要从事动物遗传育种与繁殖研究;E-mail:ymjmsj0618@yahoo.com.cn 动 物 学 研 究 2011,Aug. 32(4): 386−390 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI:10.3724/SP.J.1141.2011.04386 Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis YUE Ming-Jin1,3, MO Sai-Jun2, SONG Ping3,*, GONG Yan-Zhang1,* (1. College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China; 2. College of Basic Medical, Zhengzhou University, Zhengzhou 450001, China; 3. Laboratory of Molecular Genetics and Developmental Biology, Wuhan University, Wuhan 430072, China) Abstract: Zebrafish (Danio rerio) Z-OTU, containing OTU and TUDOR domains, was predicted to be a member of OTU-related protease, a family of the deubiquitylating enzymes (DUBs). A previous report from our laboratory clearly describes the expression patterns of z-otu mRNA. Here, we characterized the Z-OTU protein during zebrafish oogenesis and early embryogenesis. After prokaryotic expression, the recombinant protein

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