石斛兰“绿色星辰×出水芙蓉”杂交种子无菌萌发与组培快繁研究

作者:符岸军;李娟玲;李劲松;张勇;刘国民 刊名:农业科学与技术:英文版 上传者:王惠英

【摘要】[目的]研究石斛兰"绿色星辰×出水芙蓉"杂交种子无菌萌发与组培快繁。[方法]以"绿色星辰"为母本,以"出水芙蓉"为父本进行杂交,对其杂交种子进行了离体培养和组培快繁研究。[结果]石斛兰杂交种"绿色星辰×出水芙蓉"的F1代种子在改良Knudson培养基上(附加1.0mg/L6-BA、1.0mg/LNAA和10%熟香蕉泥)可获得理想的无菌萌发效果,且原球茎在该培养基上也能较快增殖。以1/2MS最适合于该杂种石斛兰F1代无菌实生苗的组培快繁;8种供试培养基用于增殖培养的效果从优到劣依次为:1/2MS〉MS〉1/3MS〉1/4MS≈N6〉改良Knudson≈B5〉H。NAA用于杂种石斛兰"绿色星辰×出水芙蓉"F1代无菌实生苗的生根壮苗效果要优于IBA;NAA的推荐浓度为2.0mg/L。[结论]为今后的新品种选育提供了育种材料。

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Agricultural Science Copyright⑥ 2010, & Technology,2010, nformation lnstitute of 11(9—10):201—204 HAAS.Al rights reserved Agricultural Biotechnology Sterile Germination of the Hybrid Seeds of[Den. Burana Green Star x Den.Rainbow-compactum] and Rapid Propagation of F1 Plantlets via Tissue Culture FU An-jun’,LI Juan—ling ,LI Jing-song’,ZHANG Yong ,LIU Guo-min 1.Sanya Science and Technology Academy for Crop W inter Multiplication,Sanya 572000;2.Kudingcha Research Institute of Hainan Univer‘ sity,Haikou 570228 Abstract 『Ob ] f . ..iective This study was to germinate the hybrid seeds of Den Burana GreeD Star x Den Rainbow-compactum under aseptic condition and to explore the parameters for rapid propagation of F plantlets via tissue culture.f Method Hybridization between Den.Burana Green Star(female parent)and Den.Rainbow-compactum(male parent)was performed and the in vitro culture and proliferation of F hybrids were studied using eight diferent basic media including MS,1/2MS,1/3MS,1/4MS,B ,N6,modified Knudson and H.j Result 1 lmproved Knud- son medium appended with 1.0 mg/L6·BA.1.0 mg/L NAA and 1O% mature banana puree performed besl in F seed germination under aseptic condition.as wel as the rapid propagation of protocorm.1ike body.Of alf the eight media lested.1/2MS is the medium most suitable for the /n vitro rapid propagation of the F seedlings.Eficiency of eight media in the/n vitro rapid propagation was in order 1/2 MS > MS>1/3 MS>1/4 MS —N > improved Knud

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