Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone

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【标题】Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone

【作者】 Jayachandran Venkatesan  Baboucarr Lowe  Panchanathan Manivasagan  Kyong-Hwa Kang  Elna Paul Chalisserry  Sukumaran Anil  Dong Gyu Kim  S-K Kim 

【关键词】Salmon MSC XRD NHA Electron 

【摘要】Nano-Hydroxyapatite (nHA) was isolated from salmon bone by alkaline hydrolysis. The resulting nHA was characterized using several analytical tools; including thermogravimetric analysis (TGA); Fourier transform infrared spectroscopy (FT-IR); X-ray diffraction analysis (XRD); scanning electron microscopy (SEM) and transmission electron microscopy (TEM); to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous. Microscopy demonstrated that the isolated nHA possessed a nanostructure with a size range of 6-37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) and was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. These results suggest that nHA derived from salmon is a promising biomaterial in the field of bone tissue engineering.