超高效液相色谱法同时测定饮料中5种人工合成色素

作者:张婉;王覃;杜宁;周悦;李津廷;张经华 刊名:食品科学 上传者:冯超

【摘要】目的:建立超高效液相色谱同时测定饮料中5种人工合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝)的检测方法。方法:样品采用聚酰胺吸附法提取色素,通过WatersACQUITYUPLCTMBEHC18反相色谱柱分离,以甲醇-0.02mol/L乙酸铵缓冲溶液为流动相进行梯度洗脱,于254nm和629nm波长处检测。结果:5种人工合成色素在5.5min内完成分离,3~80mg/L范围内呈良好线性关系(r>0.9995),检出限为0.03~0.10mg/L,平均回收率为97.4%~107.3%,RSD为0.22%~3.20%。结论:该方法简便、快速、分离效果好、灵敏度高,适用于饮料中人工合成色素的测定。

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177※分析检测 食品科学 2011, Vol. 32, No. 04 超高效液相色谱法同时测定饮料中5种人工合成色素 张 婉,王 覃,杜 宁,周 悦,李津廷,张经华* (北京市理化分析测试中心,北京 100089) 摘 要:目的:建立超高效液相色谱同时测定饮料中5 种人工合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮 蓝)的检测方法。方法:样品采用聚酰胺吸附法提取色素,通过Waters ACQUITY UPLCTM BEH C18 反相色谱柱分离,以甲醇-0.02mol/L 乙酸铵缓冲溶液为流动相进行梯度洗脱,于254nm 和 629nm 波长处检测。结果:5 种人工合成色素在5.5min 内完成分离,3~80mg/L 范围内呈良好线性关系(r >0.9995),检出限为0.03~0.10mg/L,平均回收率为97.4%~107.3%,RSD 为 0.22%~3.20%。结论:该方法简便、快速、分离效果好、灵敏度高,适用于饮料中人工合成色素的测定。 关键词:超高效液相色谱法;人工合成色素;饮料 Determination of Five Synthetic Pigments in Beverage by Ultra Performance Liquid Chromatography ZHANG Wan,WANG Tan,DU Ning,ZHOU Yue,LI Jin-ting,ZHANG Jing-hua* (Beijing Center for Physical and Chemical Analysis, Beijing 100089, China) Abstract :Objective: To develop an ultra performance liquid chromatography (UPLC) method for the determination of five synthetic pigments such as tatrazine, amaranth, carmine, sunset yellow and brilliant blue in beverage. Methods: Synthetic pigments in beverage were extracted by polyamide adsorption method, separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate, and detected at 254 nm and 629 nm. Results: The five synthetic pigments were separated in 5.5 min. The UPLC method exhibited excellent linearity over a range of 3-80 mg/L (r > 0.9995). The detection limit, recovery rate and relative standard deviation (RSD) of this method were 0.03-0.10 mg/L, 97.4%-107.3%, and 0.22%-3.20%, respectively. Conclusion: The a

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