鼓槌石斛种子萌发培养与小苗组培快繁技术研究(英文)

作者:蓝玉甜;刘世勇;罗玉婷;黄岚;李柱林;韦小路 刊名:Agricultural Science & Technology 上传者:林金华

【摘要】[目的]利用鼓槌石斛蒴果,通过无菌播种萌发发育成苗及小苗快繁技术试验研究,从而获得大量种苗。[方法]用鼓槌石斛种子通过无菌播种获得大量原球茎,分别转接到4组不同的诱导芽培养基上,筛选出鼓槌石斛最适合小苗分化和成长的培养基,以其设置以N6为基本培养基,添加浓度均为1.0、1.5、2.0mg/L的NAA和IAA,筛选最适合鼓槌石斛小苗生根壮苗培养基。[结果]将鼓槌石斛种子在MS+6-BA1mg/L+10%香蕉汁+20g/L蔗糖+6g/L琼脂+1g/LAC培养基上进行培养种子,种子萌发率达90%以上;最利于原球茎分化的培养基为N6+NAA2mg/L+BA0.5mg/L+10%香蕉汁+20g/L蔗糖+0.5g/L牛肉蛋白胨+5.8g/L琼脂+0.5g/LAC,培养出的苗整齐度高,均匀;最佳生根壮苗培养基为N6+NAA1.5mg/L+10%香蕉汁+20g/L蔗糖+5.8g/L琼脂+1g/LAC,苗生根数多、粗壮、整齐,叶浓绿。[结论]该研究结果为鼓槌石斛快速繁育技术提供了参考。

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DendrobiumchrysotoxumLindlisDendrobiumepiphyticplantsofOrchidaceae,itisanimportantornamentalandmeanwhileakeyparentmaterialofacommercialChineseTraditionalMedicine-Dendrobium.InChina,itmainlydistrib-utesintheprovinceslikeYunan,GuangxiandSichuan[1-2].Withfreshstemordriedstemasmedicine,D.chrysotoxumisakindofconventionalmedicinalDendrobiuminciviluse.Apartfromtheefficaciesofthestomachbenefitingandfluidsincreasing,andheat-clearingandYin-nourishing,D.chryso-toxumcanalsobeusedforcuringimpairmentoffluid,drymouthandtongue,heatposttodisease,poorvision[3].Be-sidesthis,itsdriedstemisabundantinbioactivecomponentsofanti-tumor[4-6].WiththeincreasingmedicinalrequirementofD.chrysotoxumandthedailyreducingwildresourcesinChina,wildplantsarefarfrompeoplesrequirement.Tomeettherequirementandmeanwhileprotectwildresourcesinasustainabledevelopmentapproach[7],anartificialcultivationculturetechniqueisurgentlyrequiredforprovidingmaterials.Inviesofthis,weperformedthestudyontherapidpropaga-tiontechniqueinD.chrysotoxum,aimingatprotectingthewildD.chrysotoxumresourcesandalsoprovidingcredibletechnicalguidanceforitsindustrializedcultivation.MaterialsandMethodsMaterialsDuringflorescenceperiod,bionics-cultivatedD.chryso-toxumplantsinYachangOrchidsNatureReservewereasma-terialsforartificialpollination.Sixmonthsafterfertilization,thecapsulesbecamecompletelymature,thentheywereusedasexplantsforsterilegermination.MethodsSurfacedisinfectionofexperimentalmaterialsThema-turecapsulesfortestwerefirstwashedwithtapwater,andbrus

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