Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing

作者:Masaya;Yamamoto;Yoshihiro;Handa;Hiroki;Aihara;Hiroaki;Setoguchi 刊名:植物多样性:英文版 上传者:雪雁鸣

【摘要】Primula reinii (Primulaceae), a perennial herb belonging to the Primula section Reinii, occurs on wet, shaded rocky cliffs in the mountains of Japan. This threatened species comprises four varieties; these plants are very localized and rare in the wild. In this study, 43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critically endangered primroses. We developed novel microsatellite markers for three varieties of R reinii, and tested its polymorphism and genetic diversity using natural populations. These novel markers displayed relatively high poly- morphism; the number of alleles and expected heterozygosities ranged from 2 to 6 (mean - 3.2) and 0.13 to 0.82 (mean = 0.45), respectively. All loci were in Hardy-Weinberg equilibrium. These microsatellite markers will be powerful tools to assess P. reinii genetic diversity and develop effective conservation and management strategies.

全文阅读

Plant Diversity 40(2018)41-44 Ke AO、,AhICING RESEJ RCH EV OLVlNG SCI ENCE , COntents Iists available at S~enceDirect 。 P1,ant Diversity jou rnal homepage:http:/, 静aipublishing.com/entjouf 1a 寮,pIant—dilv ~sity/ ∞瓤$httP:11jou rnaI.kib.ac ca - ”ill 一 Development and characterizatiOn of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing Masaya Yamamoto ’,Yoshihiro Handa b , Hiroki Aihara b . Hiroaki Setoguchi Graduate School ofHuman and Environmental Studies,Kyoto University,Yoshida Nihonmatsu.Sa 0一ku,Kyoto 606-8501.Japan 。FASMAC Co.,Ltd..5-1—3 Midorigaoka,Ats , Kanagawa 243—0041,Japan Cross]Ⅵark A R T I C L E I N F o Ar de history: Received 23 MIarch 2017 Received in revised folrm 3 September 2017 Accepted 6 September 2017 Available online 14 September 2017 ('Editor:Xun Gong) Keywords: MEicrosatellites Polymorphism MiSeq Critically endangered plant Pr/imula re{n“ A B S T R A C T Pn'mu, reinfi’(Primulaceae),.a perennial herb belon ng to the Primu, section Remi.occurs on wet. shaded r0c cliffs in the mountains of Japan.This threatened species comprises four varieties;these plants are very localized and rare jkn the wild.In this study,43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critcally endangered primroses.We developed novel microsatellite markers for three varieties of re/n//,.and tested its polymorphism and genetic diversi,ty u

参考文献

引证文献

问答

我要提问