人参皂苷Rg1对视网膜氧化应激损伤的保护作用

作者:曹妍群;黄凯;罗特坚 刊名:神经解剖学杂志 上传者:仇强

【摘要】目的:研究人参皂苷Rg1对视网膜氧化应激损伤的保护作用及可能机制。方法:在大鼠玻璃体腔内注射5μl氯化铁溶液制成视网膜氧化应激模型,连续14 d每日尾静脉注射人参皂苷溶液,单纯模型组不予处理,给药溶媒对照组等量注射生理盐水。处死取材后对大鼠视网膜采用Western Blot检测BACE1以及β-CTF含量,ELISA检测Aβ1-40的含量,通过吸光光度法检测丙二醛(MDA)含量。结果:给药处理组与单纯模型组及给药溶媒对照组相比,BACE1表达量明显降低,Aβ1-40和β-CTF产生量明显减少,丙二醛含量下降(P<0.05)。结论:人参皂苷Rg1对视网膜氧化应激损伤有保护作用,其作用机制可能与抑制大鼠视网膜BACE1表达上调并相应减少Aβ1-40、β-CTF的产生有关。

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神 经 解 剖 学 杂 志 Chinese Journal of Neuroanatomy 人参皂苷 Rgl对视网膜氧化应激损伤的保护作用 曹妍群 ,黄 凯 ,罗特坚 (1.邵阳医学 高等专科学校人体解剖教研室 ,邵阳 422000;2.中南大学湘雅 医学院人体解剖学与神经生物学系,长沙 410013) 【摘 要】 目的:研究人参皂苷 Rgl对视网膜氧化应激损伤的保护作用及可能机制。方法:在大鼠玻璃体腔内 注射 5 l氯化铁溶液制成视网膜氧化应激模型,连续 14 d每 日尾静脉注射人参皂苷溶液 ,单纯模型组不予处理, 给药溶媒对照组等量注射生理盐水。处死取材后对大鼠视网膜采用 Western Blot检测 BACEI以及 3-CTF含量, ELISA检测 A131-40的含量,通过吸光光度法检测丙二醛(MDA)含量。结果:给药处理组与单纯模型组及给药溶 媒对照组相比,BACE1表达量明显降低,A J31-4O和 p—CTF产生量明显减少,丙二醛含量下降(P<0.05)。结论: 人参皂苷 Rgl对视网膜氧化应激损伤有保护作用,其作用机制可能与抑制大鼠视网膜 BACE1表达上调并相应减 少 A131-40、B.CTF的产生有关。 【关键词】 人参皂苷 Rgl;氧化应激 ;BACEI;AB;视网膜 10.16557/j.cnki.1000-7547.2015.04.0025 The protective effect of Ginsenoside on the oxidative afress injury of rats’retina Cao Yanqun ,Huang Kai ,Luo Tejian (1.Department of Anatomy,Shaoyang Medical College,Shaoyang 422000;2.Department of Anatomy and Neumbiology,Xiangya School of Medicine,Central South University,Changsha 410013,China) 【Abstract】Objective:Our present study aims at question whether Ginsenoside shows the protective effect of retinal oxi— dized.Methods:Rats in oxidized model group were injected with 5 solution of FeC13 into vitreous body,and treatment group received Ginsenoside for 14 days after FeC13 injection,equal amount of normal saline were administered se~ed as the vehicle group.Western Blot and ELISA assay were applied to evaluate the amount of BACE1,8一CTF and A311-40. The level of malonaldehyde(MDA)was determination by the spectrophotometrie method to assess the eect of FeC13 and therapeutic agents of Ginsenosi

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