CTLA-4基因多态性与膀胱癌的易感性研究

作者:刘贵喜;李家兵 刊名:现代泌尿外科杂志 上传者:胡意钗

【摘要】目的探讨细胞毒性T淋巴细胞相关抗原4(CTLA-4)CT60G/A、-1661A/G位点单核苷酸多态性(SNPs)与膀胱癌遗传易感性及临床特征关系。方法收集四川东北部地区汉族人群病例组241例,对照组326例;采集研究对象外周静脉血4mL,行全血DNA提取、目的基因片段扩增,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对CTLA-4CT60G/A、-1661A/G多态位点进行基因型分析,明确两组中CT60G/A、-1661A/G多态位点基因型及等位基因分布情况,并采用统计学方法探讨CT60G/A、-1661A/G多态位点与膀胱癌易感性及临床分期、分级、转移之间关系。结果 CT60G/A、-1661A/G位点基因型频率分布差异有统计学意义(P〈0.05);Logistic回归分析显示。对于CT60G/A,GG使膀胱癌患病风险增加1.239倍,P=0.017,不同基因模型与膀胱癌临床分级、分期、转移之间无相关性(P〉0.05);对于-1661A/G,AG+GG使膀胱癌患病风险增加1.696倍,P=0.014;G等位基因较A等位基因显著增加膀胱癌发病风险(P=0.007);显性模型分别与膀胱癌分期和转移有统计学意义(P〈0.05)。结论 CTLA-4-1661A/G多态位点与四川东北部地区汉族人群膀胱癌遗传易感性相关,AG/GG基因型可能与膀胱癌的分期、转移相关;尚未发现CTLA-4CT60G/A多态位点与膀胱癌遗传易感性相关。

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268 J Mod Urol,Vo1.23 No.4 Apr.2018 · 临床 研 究 · CTLA一4基 因多态性与膀胱癌的易感性研究 刘贵喜 ,李 家兵 (1_西南医科大学 l临床 医学 院 ,四川泸州 646000;2.绵 阳市第三人民医院 ,四川绵 阳 621000) Association of CTLA一4 polymorphism with susceptibility to bladder cancer I IU Gui—xi 。LI Jia—bing (1.Clinical M edical College of Southwest M edical University,Luzhou 646000;2.the 3rd People’s Hospital of M ianyang,M ianyang 621000,China) ABSTRACT:Objective To investigate the relationship between single nucleotide polymorphisms(SNPs)of cytotoxic lym- phocyte antigen 4(CTLA一4),CT60 G/A and一1661 A/G sites and the genetic susceptibility to bladder cancer and the clinical characteristics.Methods A total of 241 bladder cancer cases and 326 healthy controls from the Han population in northeast- ern Sichuan Province were involved in the case—control study.In each object,4mL peripheral venous blood was collected,the DNA was extracted。and the target gene fragment was amplified.The genotypes of CTLA-4 CT60 G/A and—l661A/G were an— alyzed with polymerase chain reaction restriction fragm ent length polymorphism (PCR—RFLP).The relationship between CT60G/A and-1661A/G polymorphism and susceptibility to bladder cancer,clinical staging,grading and metastasis were ana— lyzed.Results The frequency of CT60G/A and一1661A/G genotypes showed a significant difference(P< O.05).Logistic re— gression analysis showed that CT60G/A:GG could increase the risk of bladder cancer by 1.239 times(P一0.017).There was no correlation between different gene models and the clinic

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