The effects of type beta transforming growth factor on proliferation and epidermal growth factor receptor expression in a human glioblastoma cell line

作者:Eirik Helseth; Geirmund Unsgaard; Are Dalen; Randi Vik 刊名:Journal of Neuro-Oncology 上传者:王立恒

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Journal of Neuro-Oncology 6:269-2"/6 (1988) © Kluwer Academic Publishers - Printed in the Netherlands 269 The effects of type beta transforming growth factor on proliferation and epidermal growth factor receptor expression in a human glioblastoma cell line Eirik Helseth, Geirmund Unsgaard 1, Are Dalen 2 and Randi Vik qnstitute of Cancer Research, Department of Neurosurgery; 2Department of Virology, University of Trondheim, Tronheim Regional Hospital, N-7000 Trondheim, Norway Key words: glioma, growth factors, receptors,, autocrine growth Summary Type beta transforming growth factor (B-TGF) is a potent growth inhibitor to many human tumor cell lines. Very little is known about the mechanism for this growth inhibitory action of B-TGE We here report the effect of B-TGF on proliferation and epidermal growth factor receptor (R-EGF) expression in a human glioblastoma cell line named T-MG1. B-TGF inhibite the soft agar growth of T-MG1 cells. Maximum inhibition was 70%, achieved with 0.5 units B-TGE B-TGF had no effect on monolayer growth of T-MG1 cells. T-MG1 cells contained abundant R-EGF, which could be divided into two subpopulations, one high affinity and one low affinity population of R-EGF. Treatment with B-TGF caused an initial decrease (0- 6 h) in EGF- binding, followed by an increase in EGF-binding which reached maximum after 24 h exposure to B-TGF. Since addition of EGF to agar cultures gave no additional increase in inhibition by B-TGF and EGF alone had no inhibitory effe

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