发育早期暴露氟西汀对斑马鱼社交行为发展的影响

作者:文竹;李强;杜亚松;郭宁 刊名:中国儿童保健杂志 上传者:刘连珍

【摘要】目的通过研究早期暴露于氟西汀的斑马鱼,探索氟西汀对斑马鱼五羟色胺系统及远期社交功能的影响。方法用氟西汀早期慢性处理斑马鱼(3-6dpf),通过高效液相色谱法(HPLC)检测斑马鱼脑部的主要神经递质及部分代谢产物水平;用社交偏好实验评估斑马鱼社交行为的远期发展(30dpf)。结果随氟西汀剂量的增加,五羟吲哚乙酸(5-HIAA)水平不断升高,而五羟色胺(5-HT)、多巴胺(DA)及3,4-二羟苯酰乙酸(DOPAC)在幼鱼(7dpf)脑内水平先增后减。在社交偏好实验结果中,对照组在毗邻鱼群的区域内总游动距离显著高于4μM组和8μM处理组(P均<0.01);游动时间的比较显示,对照组高于4μM组和8μM组(P均<0.05)。结论斑马鱼早期暴露于氟西汀,可能通过改变5-HT水平和功能,对神经发育过程产生持续作用,继而影响到其社交能力。

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CJCHCDEC. 23,N0.12 1267 1 3 5-HT* Q95-33 2015) 12-1267-05 doi: 11852/zgetbjzzZ015-23-1t-11 Eftect.s on long-lasting social-related behavior in early postnatal fluoxetine treated on zebrafish. WEN Zhul•u Qiang' ,GUO , 1 Shanghai Mental Health Center Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200030 China; 2 School of Medicine ,Shanghai Jiaotong University ,Shanghai 200025,China; 3 Paediathics Hospital Affiliated to Fudan University Shanghai 201102,China; 4 Shanghai University of Ihaditional Ginese Medicine,Shanghai 201203,O/ina Corresponding author DU Ya—song 163. Com Abstract: Objectives To expolore the effects toserotanin level and long-term social-related behaviour via transient early postnatal fluoxetine exposure on zebarafish larves. Methods The level of neurotransmitters were exmained and their respective metåbölites were quantified by H PLC fram whole brain Of larves days post fertization) exposure to fluoxe— tine as well as tested the long-term social—related behaviour on zebrafish (30 days post fertization)quantified by social prefer— ence test. Results The level of 5—HIAA increased by enhancing dosage of fluoxetine, whlie the level of 5—HT, DA, DOPAC increased in low does and deceased in high dose. In social preference test, compared to the control group (1 255, 50 ± 117. 95)cm,the 4 '1M group (7 IS. 20±135. 48) cm and 8 BM group (604. 66±81. 16) cm swam more distance in the area adjacent to the fish 01) ;compared to the control grot_lp (1

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