慢性血栓栓塞性肺动脉高压实验动物模型的建立

作者:贾一新;李简 刊名:中国临床康复 上传者:涂秋平

【摘要】目的:建立操作简便,成功率高,接近人体生理学的慢性血栓栓塞性肺动脉高压的动物模型。方法:①实验于2004-12/2005-03在北京大学第一医院动物实验中心完成。选用健康杂种犬12只。②给予健康杂种犬戊巴比妥静脉麻醉后,取血50mL,加入凝血酶静置制备成血栓。切开颈外静脉插入7F导管,经导管向颈外静脉内注射自体血栓适量,同时监测生理指标。③达到实验标准后,缝合静脉切口及皮肤切口,给予抗生素,动物饲养15,30,60d后,重复上述麻醉和注栓实验步骤。所有动物分别于第一次注栓前和注栓后第90天记录平均肺动脉压、肺毛细血管嵌压、心输出量、呼吸频率、潮气量、气体流速、胸内压。肺血管阻力=(平均肺动脉压-肺毛细血管嵌压)/心输出量。采用Amdur和Mead方法利用呼吸频率、潮气量、呼吸流速和胸内压计算肺气道阻力和肺动态顺应性。内皮素1水平测定用放射免疫非平衡法。④采用实验动物模型建立前后平均肺动脉压、肺血管阻力、肺气道阻力和肺动态顺应性,以及血浆内皮素1水平作为判定模型建立成功与否的标准。⑤显著性比较用配对t检验。结果:①动物模型建立成功率为83%(10/12)。②成活动物饲养第90天血浆内皮素1水平、肺动脉平均压、肺血管阻力、肺气道阻力明显高于注栓前犤(35.3±7.2)pg/L,(28±5)mmHg,(5.71±0.68)(mmHg·min)/L,(128.47±34.32)Pa/(L·s);(20.6±6.3)pg/L,(12±2)mmHg,(2.71±0.23)(mmHg·min)/L,(61.78±5.88)Pa/(L·s),P<0.05犦,肺动态顺应性明显低于注栓前犤(1.23±0.36),(1.68±0.20)mL/kPa,P<0.05犦。结论:用自体血栓连续注射法建立慢性血栓栓塞性肺动脉高压动物模型有较高的可行性。

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中国临床康复第9卷与}37期2005—08—21出版 Chinese Journal of Clinical Rehabilitation,A ugust 21 2005 V01.9 No.31 慢性血栓栓塞性肺动脉高压实验动物模型的建立★ 贾一新,李简 119 ·基础研究· 北京大学第一医院胸外科,北京市 100034 贾一新★,男,1975年生,辽宁省沈阳市人,汉族,2005年北京大学毕业,硕士,主要从事心胸外科疾病治疗的研究。Jason.Jia@163.com 中图分类号:R563 文献标识码:A文章编号:1671—5926(2005)31—0119—03 收稿13期:2005—06-05 修回日期:2005—08—04 (04/N14/YQ) Establishment of the experimental animal models of chronic thromboembolic pulmonary hypertension JJa Yi—xin,Li Jian. Department of Chest Surgery,First Hospital,Peking University,Beijing 10()034.China Jia Yi—xin★,Master,Department of Chest Surgery,First Hospital.Peking University,Beijing 100034,China Jason.Jia@163.com Received:2005-06-05 Accepted:2005—08—04 Abstract AⅣ:To establish the experimental anitaal models witll chronic thromboembolic pulmonary hypertension (CTEPH), which has simple operation.high Successful rate and similar to human physiology. METHoDS: (1)The experiment was done at the Center of Animal Experiment, First Hospital,Peking University from December 2004 to March 2005.Twe]ve healthy hybrid dogs were selected.②The 50 mL blood was gained after intravenous anesthesia with pentobarbital.The thrombus was made statically after adding the thmmbase.The 7 F tube was insertcd into extemal iugnlar vein after cutting the vein,and through the tube the suitable autologous thrombus was iniected into the cxternal jugular vein,meanwhile,to monitor the physiological index.③After reaching the experimental standard,the vein cut and skin cut were sutured

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