高糖高脂联合低剂量STZ诱导版纳微型猪2型糖尿病动物模型的建立

作者:肖国华;张素君;余坚;高天舒;王月婷;张亚丽;王宗保 刊名:中南医学科学杂志 上传者:顾海东

【摘要】目的用云南版纳微型猪建立一种新的2型糖尿病模型,探讨高糖高脂联合链脲佐菌素(STZ)对糖、脂代谢及肝、肾、胰组织结构的影响。方法高糖高脂联合低剂量STZ诱导云南版纳微型猪2型糖尿病模型,观察其血糖、胰岛素、血脂的变化,并对其肝、肾、胰组织结构的病理改变进行研究。结果喂养12月后,与正常对照组比较,模型组空腹血糖值明显升高,血清总胆固醇、甘油三酯水平升高(P<0.05),胰岛β细胞受损,脂肪变性明显,胰岛数量明显减少,胰岛内细胞数目亦减少,胞核浓染、缩小,红染加深;肝脏脂肪样变性,肝细胞索排列紊乱,肝细胞肿胀呈气球样变,肾小球肥大,硬化,肾小球内细胞增多,空泡变性。结论高糖高脂联合STZ可诱导版纳微型猪发生胰岛素抵抗、糖尿病,既表现2型糖尿病的特征,又表现1型糖尿病的特征,成功模拟了人类2型糖尿病的发病过程。

全文阅读

2012 47 40 - -0351-06 2 2.4 (1. 421001;2. 12 h % , $ < O. 05) , AS ß , Establishment of Model of Type 2 Diabetes in Chinese Banna Miniature Pigs Induced by High Sucrose/High Fat Diet and STZ XIAO Cuohua,ZHANG sujun , YU Jian,et al ( School of Medicine , University of South China , Hengyang , Hunan 421001 , China ) Abstract: Objective To establish a new animal model of the human type 2 diabetes mellitus and investigate the effects of high sucrose/high fat diet and SIZ on glucose , lipid metabolism and organizational structure of liver , kidney , pancre- as in Chinese banna I miniature pigs. Methods Minipigs were induced by high sucrose/high fat diet and SIZ and the lev- els of fasting blood glucose, insulin, total cholesterol , triglycerides were measured. Organizational structure of liver, kidney and pancreas were observed by Hematoxylin eosin staining and scanning electron photomicrographs in the minipigs. Results fie fasting plasma glucose, triglyceride and total cholesterol of model group increased significantly compared to that of control group at the end of 12 months (P 05) . Injured pancreatic ß-cells were observed, and the pancreatic islets from the model group became atrophied, with loss in number of the islets and cells within them. 'Ihe nucleus of islet cells were small and deep — dyed. There was an abundance of large vacuolations and arrangement of liver cell cord in disorder in cytoplasm. He- patic steatosis, as well as glomerular hypertrophy and glomerulosclerosi

参考文献

引证文献

问答

我要提问