B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis in Drosophila male meiosis

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【标题】B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis in Drosophila male meiosis

【作者】 Daisuke Hayashi  Karin Tanabe  Hiroka Katsube  Yoshihiro H Inoue 

【关键词】Mitosis Lamin Meiosis NPC Microtubule 

【摘要】In higher eukaryotes; nuclear envelope (NE) disassembly allows chromatin to condense and spindle microtubules to access kinetochores. The nuclear lamina; which strengthens the NE; is composed of a polymer meshwork made of A- and B-type lamins. We found that the B-type lamin (Lam) is not fully disassembled and continues to localize along the spindle envelope structure during Drosophila male meiosis I; while the A-type lamin (LamC) is completely dispersed throughout the cytoplasm. Among the nuclear pore complex proteins; Nup107 co-localized with Lam during this meiotic division. Surprisingly; Lam depletion resulted in a higher frequency of cytokinesis failure in male meiosis. We also observed the similar meiotic phenotype in Nup107-depleted cells. Abnormal localization of Lam was found in the Nup-depleted cells at premeiotic and meiotic stages. The central spindle microtubules became abnormal and recruitment of a contractile ring component to the cleavage sites was disrupted in Lam-depleted cells and Nup107-depleted cells. Therefore; we speculate that both proteins are required for a reinforcement of the spindle envelope; which supports the formation of central spindle microtubules essential for cytokinesis in Drosophila male meiosis.

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