铁皮石斛人工杂交育种快繁技术(无全文)

作者:张志勇 刊名:福建农业科技 上传者:

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【摘要】通过人工授粉获得铁皮石斛蒴果,再以蒴果种子为材料进行组培快繁试验,结果表明:人工杂交育种结实率达83%。经种子萌发诱导培养、原球茎增殖培养和壮苗生根培养等试验的结果表明:种子萌发最佳培养基为1/2MS+蛋白胨2 g/L,发芽率达到95%;原球茎增殖最佳培养基为1/2MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+15%马铃薯,增殖系数达到5.7;壮苗生根最佳培养基为1/2MS+NAA 0.5 mg/L+15%香蕉,苗高7.2 cm、根数5.6条;组培苗移栽成活率达90%以上。

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88 旅t舛艘 2011年第6期 铁皮石斛人工杂交育种快繁技术 张志勇 (福建省龙岩市农业科学研究所 364000) 摘 要 :通过人工授粉获得铁皮石斛蒴果,再 以蒴果种子为材料进行组培快繁试验,结果表 明: 人工杂交育种结实率达 83%。经种子萌发诱导培养、原球茎增殖培养和壮苗生根培养等试验的结 果表明:种子萌发最佳培养基为 1/2MS+蛋白胨 2 g/L,发芽率达到95%;原球茎增殖最佳培养 基为 1/2MS+6一BA 1.0 rng/L+NAA 0.5,ng/L+15%马铃薯,增殖系数达到 5.7;壮苗生根 最佳 培养基为 1/2MS+NAA 0.5 mg/L+15%香蕉 ,苗高 7.2 cm、根数 5.6条 ;组培苗移栽成 活率达 90% 以上 。 关键词:铁皮石斛;杂交育种;组培快繁 ;移栽 Rapid propagating techniques of artificial cross breeding of Dendrobium candidum ZHANG Zhi—yong (Longyan Institute of Agricultural Sciences,Fujian Province 364000) Abstract:An experiment of rapid tissue—culture propagation on Dendrobium candidum seeds were conducted,that capsule have been gained through artificial pollination.The results showed that the seed setting rate of artificial hybrid breeding was 83% .The test results from induction of seed germination,rapid propagation of protocorm and rooting culture for strong sprouts showed that the best medium of seeding germination,protocorm rapid propagation and strong sprout rooting culture were I/2MS +peptone 2g/L.1/2MS+6一BA 1.0 mg/L+NAA 0.5 mg/L+15% potato and 1/2MS+NAA 0.5 mg/L+ 15% banana respectively,with the germination rate of 95% ,multiplication coeicient of 5.7 and seedling height of 7.2cm and root number of 5.6 piece,respectively,the transplantation survival rate were over 90% . Key words:Dendrobium candidum ;cross breeding;rapid propagation of tissue culture;transplant 铁皮石斛又名黑节草 ,为兰科石斛属多年生常 绿草本植物,是一种名贵药材,在 《道藏》中被列 为中华九大仙草之首⋯。铁皮石斛主要药用成分为 石斛多糖,具有提高人体免疫功能、抗衰老、防治 肿瘤和心血管疾

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