冬凤兰非共生萌发和低温离体保存(英文)

作者:罗远华;冷青云;莫饶;陈业渊 刊名:Agricultural Science & Technology 上传者:李志明

【摘要】[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource.

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Cymbidiumdayanumisakindofwildepibiosisorchidswithdarkgreenstripleaves,racemeandgoodplanttype,whichshowsagreatvalueofexploitationandutilization.ButimproperexploitationmethodshavecausedagreatlossofwildC.dayanumresourcesanditsnaturaldistributionhasdecreaseddramatically.Soitisnecessarytostudytheinvitroconservationofprotocormsatlowtemperaturebyusingplanttissueculturetechnique,whichwillhelptopreserve,exploitandutilizethegermplasmresourceofC.dayanumwell.Asymbioticgermination(asepticgermination)isamainmethodofseedgerminationofOrchidaceae.Low-temperatureinvitroconservationisoneofthemethodsmostcommonlyusedinplantgermplasmresourcepreservation.Homes[1]etal.firstlyreportedlow-temperatureconservationofCymbidi-umprotocorm.Studiesaboutinvitroconservationofgerm-plasmresourcesofCymbidium,Dendrobium,Vanilla,Spathoglattis,VandaandBletillaetc.havebeenconductedbyfar[1].Butreportsaboutasepticseedingandlow-temper-atureinvitroconservationofC.dayanumprotocormhaven’tbeenfound.Therefore,low-temperatureinvitroconservationtechniquesystemisestablishedbyusingtheprotocormsob-tainedbymatureseedsasepticgermination,whichwillpro-videasupportforgermplasmresourcepreservationofC.dayanum.MaterialsandMethodsMaterialsMatureseedsofC.dayanum.MethodsSeedsterilizationandasepticinoculationThesurfaceofmaturecapsuleswithoutsplitwerewashedbytapwaterandthelesionswereparedoffwithascalpelontheinoculatingtable,thenthecapsuleswereswabbedwith75%(V/V,thesamebelow)alcoholanddippedinto95%alcoholforseveralsecondsbeforesterilizationwith

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