Correlation between hormone dependency and the regulation of epidermal growth factor receptor by tumor promoters in human mammary carcinoma cells.

作者:Roos, W.; Fabbro, D.; Kung, W.; Costa, S. D.; Eppenberger, U. 刊名:Proceedings of the National Academy of Sciences 上传者:张必成

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Proc. Nati. Acad. Sci. USA Vol. 83, pp. 991-995, February 1986 Cell Biology Correlation between hormone dependency and the regulation of epidermal growth factor receptor by tumor promoters in human mammary carcinoma cells (breast cancer cells/phorbol ester/proliferation/protein kinase C/steroid receptor) WERNER Roos, DORIANO FABBRO, WILLY KONG, SERBAN D. COSTA, AND URS EPPENBERGER* Laboratory of Biochemistry-Endocrinology, Department of Research, and Department of Gynecology and Obstetrics, University Clinic Medical School, CH-4031 Basel, Switzerland Communicated by W. Arber, September 24, 1985 ABSTRACT The effects of the tumor promoter phorbol 12-tetradecanoate 13-acetate (TPA) on the epidermal growth factor (EGF) receptor levels were investigated in hormonedependent (MCF-7, T-47-D, and ZR-75-1) and hormoneindependent (MDA-MB-231, HBL-100, and BT-20) human mammary carcinoma cell lines. In the absence of TPA, hormone-independent cell lines contained high concentrations of low-affinity EGF receptors (apparent Kd = 8 x 1010 M), whereas hormone-dependent cell lines exhibited low concentrations of high-affinity receptors (apparent Kd = 1 x 10-10 M). TPA causes a change of the receptor from a high- to the low-affinity state in hormone-dependent cell lines (MCF-7, T-47-D, and ZR-75-1), as well as in the hormone-independent HBL-100, whereas the affinity remained unchanged in MDAMB-231 and BT-20 cells. In addition, progesterone receptor levels are decreased after TPA treatment in the

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