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【摘要】Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in affaffa nodule development.