In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane(无全文)

作者:NongYueHE JianXinTANG;SongLI HongCHEN;AnCunZHOU 刊名:中国化学快报:英文版 上传者:

抱歉,该篇全文还没有人上传哦!我要 上传, 我要 求助

【摘要】Polypropylene microporous membranes were treated with plasma in a mixture of N2and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstratedthe success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.

参考文献

引证文献

问答

我要提问