Increased TNF expression in CD43++ murine blood monocytes

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【作者】 Bernard Burke  Rasheedah Ahmad  Karl J. Staples  Roger Snowden  Aras Kadioglu  Marion Frankenberger  David A. Hume  Loems Ziegler-Heitbrock 

【关键词】Monocytes  Murine  Subpopulations  TNF 


【摘要】Monocyte heterogeneity has been studied extensively in man but only recently tools have been developed to study blood monocyte populations in the mouse. We have used the MacGreen mouse model, which expresses the green fluorescent protein under the control of the promoter of the murine M-CSF receptor (CSF1 receptor, c-fms). Since both monocytes and granulocytes show GFP expression in this model the latter cells were excluded by staining with the Ly6G granulocyte marker. GFP+ Ly6G blood monocytes were found to account for an average of 246 ± 121 cells/μl in these mice. These monocytes can be subdivided into CD43+ GR-1+ cells and CD43++ GR-1 cells, with the latter cells accounting for 140 ± 77 cells/μl, i.e. about 60%of all blood monocytes. After intraperitoneal injection of lipopolysaccharide (LPS) both blood monocyte subpopulations were depleted. The same was true after intranasal infection with Streptococcus pneumoniae but here the CD43++ subpopulation was preferentially reduced to 4 cells/μl.

【刊名】Immunology Letters